SELECTIVE TARGETING OF TUMOR VASCULATURE USING ANTIBODY MOLECULE

摘要

PROBLEM TO BE SOLVED: To provide a specific binding member binding human ED-B, containing an antigen-binding part comprising an antibody VH domain and an antibody VL domain.

SOLUTION: Provided are selectively targeting tumoral vasculature in vivo using a human recombinant scFv, L19, to the angiogenesis marker ED-B domain of fibronectin. In preferred embodiments, a complete human IgG1 having the variable regions of L19 is employed. In other embodiments a mini-immunoglobulin generated by fusing the scFv L19 to the constant CH4 domain of a secretory IgE isoform that naturally contains a cysteine in its COOH terminal and which forms a covalently linked dimer is employed. Different in vivo behavior of the antibody formats is exploitable for different diagnostic and/or therapeutic purposes, depending on clinical needs and disease. The antibody molecules may be labelled as described.

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