RECOMBINANT VECTOR EXPRESSING MULTIPLE COSTIMULATORY MOLECULES AND USE THEREOF

摘要

PROBLEM TO BE SOLVED: To provide a recombinant vector encoding or expressing at least three costimulatory molecules, further to provide a recombinant vector containing a gene encoding one or more target antigens or immunological epitope thereof.

SOLUTION: The synergistic effect of these costimulatory molecules on the enhanced activation of T cells is demonstrated. The degree of T cell activation using recombinant vectors containing genes encoding three costimulatory molecules was far greater than that of the sum of recombinant vector constructs containing one costimulatory molecule and greater than that in the use of two costimulatory molecules. Results employing the triple costimulatory vectors were most significant under conditions of either low levels of a first signal or low stimulator to T-cell ratios. This phenomenon was observed for both isolated CD4+and CD8+T cells. The recombinant vectors are useful as immunogenes and vaccines against cancer and pathogenic micro-organisms, and in providing host cells, including dendritic cells and splenocytes with enhanced antigen-presenting functions.

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