METHOD OF MANUFACTURING INDUCED PLURIPOTENT STEM CELL ORIGINATED FROM HUMAN SOMATIC CELL

摘要

Disclosed is a method for manufacturing stem cells including preparing Oct-4 gene, Sox2 gene, Nanog gene, and fourth gene from human embryonic stem cells, and allowing each of the genes to be infected in host cells using a lentiviral vector system to generate viruses in which each of the genes are induced; concentrating or mixing each of the viruses to prepare a virus concentrated mixture, and mixing the virus concentrated mixture and a first culture solution to prepare a virus solution; floating human somatic cells having been cultivated in advance in a first culture dish, and mixing and reacting the floated somatic cells and the virus solution to prepare a somatic cell- virus mixture; adding and retaining the somatic cell-virus mixture as is in a second culture dish including a second culture solution to induce the genes in the somatic cells; and cultivating the somatic cells.