| 摘要 |
The present invention provides a method of forming a three-dimensional structure of unidirectionally aligned cells. The method comprises providing a substrate with a microchannel. The microchannel is defined by at least a pair of opposing lateral walls and a base. In at least a portion of the microchannel the distance between the pair of opposing lateral walls is within the micrometer range. A first plurality of cells is seeded in the microchannel and the cells are allowed to proliferate up to at least a density of at least 90%. Thereby contact guidance cues are provided by the pair of opposing lateral walls of the microchannel, such that the cells align unidirectionally. Thereby a first layer of aligned cells is also formed at the base of the microchannel. A second plurality of cells is seeded in the microchannel, which already comprises a first layer of aligned cells. When allowing cells of the second plurality of cells to proliferate up to at least substantial confluence, contact guidance cues are again provided by the lateral walls of the microchannel. The cells also align unidirectionally and form a second layer of aligned cells.
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