| 摘要 |
Disclosed is a recombinant, eukaryotic glycosyltransferase, wherein a stem anchor region and a transmembrane domain are deleted from the recombinant, eukaryotic glycosyltransferase, and wherein the glycosyltransferase is fused in frame to a maltose binding domain. Also disclosed is a method of refolding a first insoluble, recombinant, eukaryotic glycosyltransferase, wherein the glycosyltransferase comprises a maltose binding protein domain (MBD),the method comprising the steps of (a) solubilising the insoluble, recombinant, eukaryotic glycosyltransferase in a solubilization buffer; and (b) contacting the soluble eukaryotic glycosyltransferase with a refolding buffer comprising a redox couple to refold the eukaryotic glycosyltransferase, wherein the refolded eukaryotic glycosyltransferase catalyzes the transfer of a sugar from a donor substrate to an acceptor substrate.
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