| 摘要 |
Provided is a method for the simultaneous detection of a plurality of distinct target ncRNAs, said method comprising the steps of: a. contacting a patient RNA sample containing said plurality of target ncRNAs with a first oligonucleotide specific for each of said target ncRNAs to be detected under conditions appropriate to form a complex between said first oligonucleotides and said target ncRNAs, each of said first oligonucleotides comprising a first signal generator to generate a first detectable signal and each of said first oligonucleotides having a first Tm for binding each of said target ncRNAs that is substantially the same; b. contacting said sample with a second oligonucleotide to bind for each of said target RNA to be detected under conditions appropriate to form a complex between said second oligonucleotides and said target ncRNAs, said second oligonucleotide comprising a second signal generator to generate a second detectable signal, wherein the first detectable signal is detectable in the presence of the second detectable signal, and wherein the second detectable signal is detectable in the presence of the first detectable signal, and each of said second oligonucleotides having a second Tm for binding each of said target ncRNAs that is substantially the same; and c. determining the presence of said plurality of target ncRNA in said sample by measuring the first and second detectable signals.
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