发明名称 |
A METHOD FOR DETECTING INTRACYTOPLASMIC PROTEIN/PROTEIN INTERACTIONS |
摘要 |
The present invention provides a versatile and sensitive method for studying interaction of two peptides or polypeptides A and B within the cytoplasm of a host cell. The method is based on the use of two distinct chimeric polypeptides. The first chimeric polypeptide containing an aggregation domain fused to a polypeptide A and the second one containing a phenotype-associated functional domain fused to a second polypeptide B. When these chimeric polypeptides are co-expressed within a host cell allowing aggregation of the aggregation domain, the phenotype of said host cell depends on the entrapment of the phenotype-associated functional domain which only occurs when A and B interact with each other.
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申请公布号 |
WO2007119165(A8) |
申请公布日期 |
2009.08.13 |
申请号 |
WO2007IB01010 |
申请日期 |
2007.04.18 |
申请人 |
MILLEGEN;RENAUT, LAURENCE;BOUAYADI, KHALIL |
发明人 |
RENAUT, LAURENCE;BOUAYADI, KHALIL |
分类号 |
C12N15/10;C12Q1/68;G01N33/542 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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