摘要 |
The present invention relates to methods of producing crosslinked hyaluronic acid microbeads, as well as the produced microbeads, said method comprising the steps of: (a) providing an aqueous alkaline solution comprising hyaluronic acid, or a salt thereof; (b) forming microdroplets having a desired size from the mixed solution of step (a) in an organic or oil phase to form a water in organic or water in oil (VWO) emulsion, wherein the amount of oil phase used is of from 20 to less than 50 % by weight based on the sum of oil phase and water; (c) adding a solution comprising a crosslinking agent to the emulsion, whereby the reaction of hyaluronic acid with the crosslinking agent takes place to provide crosslinked hyaluronic acid microbeads; and (d) optionally working up the dispersion of crosslinked hyaluronic acid microbeads obtained in step (c). |
申请人 |
EVONIK GOLDSCHMIDT GMBH;NOVOZYMES BIOPOLYMER A/S;WENK, HANS HENNING;FARWICK, MIKE;NATTLAND, SANDRA;MACZKIEWITZ, URSULA;MOELHOLM MALLE, BIRGITTE |
发明人 |
WENK, HANS HENNING;FARWICK, MIKE;NATTLAND, SANDRA;MACZKIEWITZ, URSULA;MOELHOLM MALLE, BIRGITTE |