发明名称 |
METHODS OF INDUCING OR INCREASING THE EXPRESSION OF PROTEOGLYCANS SUCH AS AGGRECAN IN CELLS |
摘要 |
Methods of inducing the expression of a proteoglycan such as aggrecan in a cell are described. A method is described which includes transfecting a cell with an isolated nucleic acid comprising a nucleotide sequence encoding a LIM mineralization protein operably linked to a promoter. The LIM mineralization protein can be rLMP, hLMP-1, hLMP-1s, or hLMP-3. Transfection maybe accomplishedex vivo or in vivo by direct injection of virus or naked DNA, or by a nonviral vector such as a plasmid. The method can be used to induce proteoglycan synthesis in osseous cells or to stimulate proteoglycan and/or collagen production in cells capable of producing proteoglycan and/or collagen (e.g., intervertebral disc cells including cells of the nucleus pulposus and annulus fibrosus).
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申请公布号 |
WO2008156500(A4) |
申请公布日期 |
2009.06.11 |
申请号 |
WO2007US84922 |
申请日期 |
2007.11.16 |
申请人 |
WARSAW ORTHOPEDIC, INC.;YOON, SANGWOOK T.;EMORY UNIVERSITY;BODEN, SCOTT D.;MCKAY, WILLIAM F. |
发明人 |
BODEN, SCOTT D.;MCKAY, WILLIAM F. |
分类号 |
C12N15/09 |
主分类号 |
C12N15/09 |
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