<p>Disclosed is a biofilm-removing agent that removes biofilms effectively. Biofilm-removing agent that includes a basic amino acid derivative represented by the General Formula (1) or a salt thereof. (1) (In the formula, R1 indicates a C4-18 straight-chain or branched alkyl or alkenyl group. X and Y indicate groups selected from groups represented by the formulas (2). m indicates an integer 1-5.)</p>