发明名称 |
PREPARING METHOD FOR (S)-3-HYDROXYBUTYRIC ACID AND (S)-3-HYDROXYBUTYRATE ESTER USING RECOMBINANT MICROORGANISM |
摘要 |
A method of synthesizing optically-active (S)-3-hydroxybutyric acid and (S)-3-hydroxybutyrate ester using a mutated microorganism is provided. More particularly, a mutated microorganism for preparing (S)-3-hydroxybutyric acid transformed with a gene encoding ß-ketothiolase, a gene encoding (S)-3- hydroxybutyryl CoA dehydrogenase and a gene encoding acyl CoA hydrolase; a method of preparing (S)-3-hydroxybutyric acid using the mutated microorganism; a mutated microorganism for preparing (S)-3-hydroxybutyrate ester transformed with a gene encoding ß-ketothiolase, a gene encoding (S)- 3-hydroxybutyryl CoA dehydrogenase, a gene encoding acyl CoA hydrolase and a gene encoding lipase; and a method of preparing (S)-3-hydroxybutyrate ester using the mutated microorganism are provided. Accordingly, (S)-3-hydroxybutyric acid with high optical purity may be produced from acetyl CoA produced in glycolysis of a microorganism by a simple process involving the manipulation of a metabolic pathway by a recombinant gene introduced into the microorganism without using a high-cost metal catalyst or a substrate. Further, (S)-3-hydroxybutyrate ester and lactone of (S)-3-hydroxybutyrate ester may be simply produced from (S)-3- hydroxybutyric acid produced by the above method using lipase. |
申请公布号 |
WO2009020279(A1) |
申请公布日期 |
2009.02.12 |
申请号 |
WO2008KR03217 |
申请日期 |
2008.06.10 |
申请人 |
LG CHEM, LTD.;PARK, SI JAE;LEE, SANG HYUN |
发明人 |
PARK, SI JAE;LEE, SANG HYUN |
分类号 |
C12N15/66 |
主分类号 |
C12N15/66 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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