| 摘要 |
A method of using electrokinetics for separating particles in a buffer solution is provided, where a chromatographic column is provided having a non-uniform internal longitudinal cross-section. At least one main inlet for inputting solution and at least one main outlet for outputting solution are provided. At least one sample inlet and at least one sample outlet are provided. The particle is introduced to the column from the sample inlet and fractionated samples are eluted from the sample outlet, where quality control and further analysis are enabled. An electric field is applied to the solution in the column to generate a charged double layer at a solid-liquid interface within the column. The electric filed moves ions within the double layer, and a non-uniform velocity profile is induced to the buffer solution. The moving ions carry the particles along the column and the particles are separated according to size or charge.
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