Recombinant expression of factor VIII in human cells

摘要

The invention discloses a process for recombinant production of blood coagulation Factor VIII in an immortalized human embryonic retina cell, said cell expressing at least an adenoviral E1A protein and comprising a nucleic acid sequence encoding said Factor VIII, said nucleic acid sequence being under control of a heterologous promoter, said process comprising culturing said cell and expressing the Factor VIII in said cell, and harvesting the expressed Factor VIII. Cells that can be used in the process of the invention are also provided.