发明名称 |
PROCESS FOR PRODUCING CELL CULTURE AND CELL CULTURE |
摘要 |
It is intended to provide a process for producing a cell culture wherein feeder cells can be efficiently removed from a cell culture formed by culturing cells. This object is established by a process which comprises the first culture step of culturing inactivated feeder cells together with subject cells to be cultured, the feeder cell-removal step of selectively removing the feeder cells from the cells after the completion of the first culture step, and the second culture step of continuously culturing the subject cells to be cultured after the completion of the removal step. Thus, the feeder cells are removed between the first culture step and the second culture step and, therefore, the content of the feeder cells in the cell culture finally obtained after the subsequent second culture step can be easily lessened. |
申请公布号 |
WO2005035734(A8) |
申请公布日期 |
2006.05.11 |
申请号 |
WO2004JP14534 |
申请日期 |
2004.09.27 |
申请人 |
JAPAN TISSUE ENGINEERING CO.,LTD.;INOIE, MASUKAZU;NINAGAWA, YOSHIHIDE |
发明人 |
INOIE, MASUKAZU;NINAGAWA, YOSHIHIDE |
分类号 |
C12N5/071;(IPC1-7):C12N5/00;C12N5/06;C12N5/08;A61L27/38;A61L27/60 |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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