METHOD FOR EXTRACTING THE NERVE TISSUE GROWTH FACTOR FROM V.LEBETINA LINNAEUS VENOM

摘要

The invention relates to producing bioactive substances and can be used for biotechnological production processes, in medicine and in the pharmacological industry, in particular to producing medicinal and diagnosis preparations from snake venom. The inventive method consists in carrying affinity chromatography prior to a fractional chromatography in two steps on a column with a Source 15S carrier by using, at a first step, a sodium carbonate solution with a pH of 6.5-6.6 in the form of a 0.19-0.21 M buffer solution and said solution with a concentration of 0.31-0.33M in the form of an eluent, in diluting 4-5 times the thus obtained eleuent with distilled water and in re-chromatographing on a column with the same carrier but less by 10 times in volume by using a sodium carbonate solution with a pH of 5.0-5.2 in the form of a 0.045-0.55 M buffer solution and the same solution with a concentration of 0.95-1.05M and a pH of 6.4-6.6 in the form of an eleuent, and in carrying out fractional chromatography of the thus obtained eluent on A Superdex 75 with a 0.19-0.21 ammonium bicarbonate solution with a pH of 6.4-6.6.