INTRACELLULAR ISOTOPE DETECTION AND USES THEREOF

摘要

A method is disclosed for measuring the hydrogen and/or oxygen isotope ratio of intracellular water in Escherichia coli cells and correlating the hydrogen and/or oxygen isotope ratio with the metabolic activity of the cell. A method is also disclosed for measuring the hydrogen and/or oxygen ratio of intracellular water via a probe, such as a fatty acid, and correlating the hydrogen and/or oxygen ratio with the metabolic activity of the cell. Methods for measuring the hydrogen and/or oxygen isotope ratio of water from eukaryotic organisms, such as cultured rat fibroblasts and whole mammals, and optionally relating the same to a metabolic rate, are also disclosed.