| 摘要 |
The invention relates to the field of drug delivery. In particular, it relates to use of probiotic bacteria, generally Gram-positive bacteria such as Lactococcus sp., in providing biologically active polypeptides in the body, especially in the gastrointestinal (GI) tract. Provided is a non-invasive microbial host cell expressing a chimeric protein comprising a therapeutic proteinaceous substance fused to a microbial membrane anchoring domain, said therapeutic proteinaceous substance comprising at least one targeting moiety capable of binding to a target cell surface molecule, and at least one bioactive polypeptide, wherein said bioactive polypeptide is flanked on both sides by at least one cleavage site for an endopeptidase activity associated with said target cell. Also provided are pharmaceutical and nutritional compositions comprising a host cell according to the invention. |
