发明名称 |
A Cyanine-based probe/tag-peptide pair for fluorescence protein imaging and fluorescence protein imaging methods |
摘要 |
A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl<SUB>3</SUB>. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.
|
申请公布号 |
US2008254546(A1) |
申请公布日期 |
2008.10.16 |
申请号 |
US20070787078 |
申请日期 |
2007.04.12 |
申请人 |
BATTELLE MEMORIAL INSTITUTE |
发明人 |
MAYER-CUMBLIDGE M. ULJANA;CAO HAISHI |
分类号 |
G01N21/00;C07D209/00;C07F9/66;C07F9/80;C07K1/13;C07K7/00 |
主分类号 |
G01N21/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|