发明名称 |
RAPID ONE-STEP REVERSE TRANSCRIPTASE PCR |
摘要 |
The present invention is directed to a method for performing a one-step RT-PCR for amplifying a target RNA comprising the steps (i) providing a sample which is supposed to contain said target RNA (ii) adding a reaction mixture comprising all reagents necessary to reverse transcribe said target RNA into cDNA and amplify at least a portion of said cDNA (iii) incubating said sample for a time interval of 0 seconds to 40 seconds at a temperature between 20° C. and 65° C., and (iv) subjecting said sample to multiple cycles of a thermocycling protocol wherein the temperature of said sample is varied between at least a first temperature between 37° C. and 72° C. and a second temperature between 85° C. and 100° C.
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申请公布号 |
US2008248535(A1) |
申请公布日期 |
2008.10.09 |
申请号 |
US20080061700 |
申请日期 |
2008.04.03 |
申请人 |
ANKENBAUER WALTRAUD;GREPL URSULA;HAERTEIS RITA |
发明人 |
ANKENBAUER WALTRAUD;GREPL URSULA;HAERTEIS RITA |
分类号 |
C12P19/34 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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