| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for fractionating an ACE-inhibiting activity fraction from germinated fermented buckwheat, by which the fraction containing an ACE-inhibiting activity ingredient is fractionated from a supernatant of the germinated fermented buckwheat to specify the ACE-inhibiting activity ingredient from the supernatant of the germinated fermented buckwheat. <P>SOLUTION: This method for fractionating a fraction prepared an angiotensin converting enzyme (ACE)-inhibiting activity ingredient from the supernatant of the germinated fermented buckwheat formed by fermenting a buckwheat bud juice, is characterized by comprising (a) processes of centrifuging the germinated fermented buckwheat and then lyophilizing its supernatant, (b) processes of dissolving the obtained lyophilized product in a water-containing alcohol solution, centrifuging the solution, and then lyophilizing the obtained supernatant, (c) processes of dissolving the lyophilized product in water, and then filtering off insolubles, and (d) a process of applying a reversed phase high performance liquid chromatography (reversed phase HPLC) to the resultant filtrate to fractionate an ACE-inhibiting activity fraction. <P>COPYRIGHT: (C)2009,JPO&INPIT |
