发明名称 |
Fluorescence Polarization-Based Homogeneous Assay For Aflatoxins |
摘要 |
A homogeneous assay for determining the aflatoxin content in agricultural products uses the technique of fluorescence polarization. A solvent is used to extract aflatoxins from a sample of the agricultural product. A mixture is prepared by combining the extract with a tracer and with a monoclonal antibody specific for aflatoxin. The tracer is able to bind to the monoclonal antibody to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating an aflatoxin oxime to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The aflatoxin concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of aflatoxin solutions of known concentration.
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申请公布号 |
US2008227221(A1) |
申请公布日期 |
2008.09.18 |
申请号 |
US20080127350 |
申请日期 |
2008.05.27 |
申请人 |
DIACHEMIX, LLC |
发明人 |
NASIR MOHAMMAD SARWAR;JOLLEY MICHAEL E. |
分类号 |
G01N33/536;G01N33/533;G01N33/542;G01N33/58;G01N33/92 |
主分类号 |
G01N33/536 |
代理机构 |
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地址 |
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