NUCLEIC ACIDS EXTENSION ASSAY

摘要

Disclosed is a method of testing for the presence of a nucleic acid se-quen ce of interest in a sample, comprising: contacting the sample with a first probe and a second probe, portions of said probes being capable of hybridis- ing to the sequence of interest such that the probes are adjacent or substan- tially adjacent to one another, so as to enable other portions of the first and second probes to become annealed to each other: treating the sample so as to cause chain extension of one of the probes using part of a probe as a tem-plate; and detecting at least part of the extended probe.