发明名称 |
PLURIPOTENT STEM CELL DERIVED FROM CARDIAC TISSUE |
摘要 |
An object of the present invention is to provide a stem cell applicable to regenerative therapeutic method, and to provide a technique to carry out regenerative therapy using the cell. A collected cardiac tissue fragment is enzymatically treated to prepare a cell suspension. Then using the cell suspension, following steps are carried out: (1) separation of cells by the density gradient method, (2) suspension-culture in a culture medium containing fibroblast growth factor and epidermal growth factor and (3) selection and separation of cells forming a floating sphere to obtain pluripotent stem cells. Thus-obtained pluripotent stem cells are used to carry out regenerative therapy. |
申请公布号 |
EP1857544(A4) |
申请公布日期 |
2008.08.13 |
申请号 |
EP20060715194 |
申请日期 |
2006.03.03 |
申请人 |
KYOTO UNIVERSITY |
发明人 |
OH, HIDEMASA;TATEISHI, KENTO;MATSUBARA, HIROAKI |
分类号 |
A61K35/34;A61L27/00;A61P9/00;A61P41/00;A61P43/00;C12N5/071;C12N5/074;C12N5/077 |
主分类号 |
A61K35/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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