| 摘要 |
Disclosed is a method for producing double-stranded DNA comprising treating double-stranded DNA having a homopolymer part or parts at one or both ends with a restriction enzyme to partly or fully eliminate at least one of the homopolymer part or parts. The restriction enzyme is capable of cleaving double-stranded DNA at a cleavage site separate from a recognition site therefor. Disclosed is a method for determining a nucleotide sequence of double-stranded DNA utilizing one or both strands of the double-stranded DNA as a template, wherein the double-stranded DNA used as the template is double-stranded DNA prepared by the above method of the present invention. The present invention provides a method for producing double-stranded DNA a part or all of which homopolymer part, which may inhibit nucleotide sequence determination, is eliminated, and a method for determining a nucleotide sequence utilizing as a template the double-stranded DNA prepared by the above method of the present invention, whose homopolymer part is partially or fully eliminated. <IMAGE> |
