| 摘要 |
It is an object of the present invention to provide a method capable of measuring a high concentration (up to 2500 U/L) of animal sample without dilutions, which is further capable of specifically measuring alpha-amylase even in a sample comprising animal alpha-amylase (EC 3.2.1.1) and glucoamylase (EC 3.2.1.3). The present invention provides a method for specifically measuring alpha-amylase contained in a non-human animal sample comprising alpha-amylase and glucoamylase without diluting said sample, wherein measurement is carried out by using an oligosaccharide having a protected nonreducing terminus and a p-nitrophenyl group at the reducing terminus thereof, and pH of reaction is 6 or more, and less than 7. |
