摘要 |
<p>A method for obtaining a stable xeno-free hBS cell line, xeno-free hBS cell lines obtained according to said method and use thereof. The method comprises the steps of: i) removing the zona pellucida from a blastocyst to obtain trophectoderm-enclosed inner cell mass by a xeno-free procedure, ii) at least partly removing the trophectoderm to obtain isolated inner cell mass cells by a xeno-free procedure, iii) placing the inner cell mass cells on a layer of human feeder cells in a xeno-free medium, iv) co-culturing of the inner cell mass cells with human feeder cells for a time period of from about 5 days to about 50 days in a xeno-free medium, v) releasing the inner cell mass cells or cells derived thereof from trophectoderm overgrowth, if any, by a xeno-free procedure, vi) selectively, transferring the inner cell mass cells or cells derived thereof to a fresh layer of human feeder cells in a xeno-free medium to obtain xeno-free hBS cells, vii) propagating the xeno-free hBS cells by co-culturing with human feeder cells in a xeno-free medium to obtain a xeno-free hBS cell line. The xeno-free hBS cell line is suitable for use in medicine and in in vitro testing.</p> |
申请人 |
CELLARTIS AB |
发明人 |
SEMB, HENRIK;STREHL, RAIMUND;HYLLNER, SVEN JOHAN;ELLERSTROEM, CATHARINA;FREJ, KATARINA;MOYA, KARINA;KILMARE, EVA KARIN |