COUPLING OF ELEMENTS

摘要

The present invention relates to a method of light-induced coupling two elements A and B via thiol binding. According to the method, molecules, proteins, peptides or peptide-containing elements can be coupled to a support or to a second molecule, protein, peptide or peptide-containing element, where the structural and functional properties of the coupled or immobilised elements are preserved, and the orientation of coupling can be controlled on a molecular scale. In particular, the method comprises generating an element A* by providing an appropriate disulfide bridge or disulfide bridge-containing triad to said element A, irradiating element A* to create an appropriate reactive thiol group from the disulfide bridge, and incubating the irradiated element A* with said element B, thereby a coupling between element A and element B is obtained. Alternatively, element A* can also be irradiated in the presence of B.