发明名称 |
Process for producing a decaffeinated coffee plant by genetic recombination |
摘要 |
The invention provides a process for producing a decaffeinated coffee plant by genetic recombination comprising a step for preparing an antisense sequence or an RNAi sequence of a gene coding for an enzyme related to the caffeine biosynthetic pathway and constructing an expression vector for transformation, a step for introducing the obtained expression vector into Agrobacterium, a step for infecting a cell division-activated tissue piece of a coffee plant or a callus or an adventitious embryo induced from a tissue piece of a coffee plant with the Agrobacterium to transform the tissue piece, the callus or the adventitious embryo and a step for obtaining an transformed coffee plant from a transformed tissue piece, a transformed callus or a transformed adventitious embryo so that a coffee plant with low caffeine content can be produced by suppressing expression of a gene coding for an enzyme related to the caffeine biosynthetic pathway by the antisense method or the RNAi method.
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申请公布号 |
US2008127373(A1) |
申请公布日期 |
2008.05.29 |
申请号 |
US20050521244 |
申请日期 |
2005.01.14 |
申请人 |
OGITA SHINJIRO;SANO HIROSHI;KOIZUMI NOZOMU;SHINMYO ATSUHIKO |
发明人 |
OGITA SHINJIRO;SANO HIROSHI;KOIZUMI NOZOMU;SHINMYO ATSUHIKO |
分类号 |
A01H5/00;C12N9/10;C12N15/09;C12N15/82 |
主分类号 |
A01H5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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