| 摘要 |
<p>A biodegradable crosslinked polyethyleneimine is obtained by reacting a linear or branched polyethyleneimine with molecular weight of 200-100000Da with a crosslinker having biodegradable ester bonds, wherein the crosslinker has molecular weight of 50-50000Da and is selected from glycidyl esters of diacid , glycidyl esters of (meth)acrylic, polyol, polyol multi-(meth)acrylates, or their mixtures. The crosslinked polyethyleneimine can be conjugated with a targeting molecule to obtain a targeting crosslinked polyethyleneimine. A method of cell transfection is accomplished by contacting a cell with the compositon comprising the crosslinked polyethyleneimine and a nucleic acid. The polyethyleneimine is used in the gene transfection reagent, the delivery system of gene therapy drug, and the carrier of drug and bioactivator such as protein and polypeptide and the like.</p> |
