| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To select MS data suitable for performing protein relative quantitative determination. <P>SOLUTION: Protein relative quantitative determination based on the MS data obtained by supplying the mixture of peptide fragments obtained from two or more proteins that have the same amino acid array and different isotope to an LC/MS/MS device is performed by using computer software. Specifically, the MS data used in determining the peptide fragments is set as a temporary reference MS data (S310), a definiteness index of each of MS data existing in a longitudinal predetermined range of the LC retention time is calculated, and the largest value is set as a reference value (S315-S340). The definiteness index of each of MS data existing before and after the LC retention time of the MS data (reference MS data) used for calculation of the reference value is calculated, and the MS data used for protein quantitative determination is selected based on the calculated definiteness index and the reference value. <P>COPYRIGHT: (C)2008,JPO&INPIT</p> |
