PROTEINS RECRUITED BY VIRUS DURING INFECTION OF PLANTS AND METHOD FOR THEIR ISOLATION AND IDENTIFICATION

摘要

The invention relates to a method for isolating and identifying proteins from protein- virus complexes and comprises: -the gel exclusion chromatography of virus-protein complexes extracted from infected plants or such as obtained by adding a purified virus to soluble proteins extracted from non infected plants, -SDS-PAGE of the proteins -analysis by NanoLC-MS/MS after tryptic digestion and protein identification.