Hybrid and tandem expression of neisserial proteins

摘要

Disclosed is a hybrid protein having formula: NH2-A-[-X-L-]n-B-COOH wherein L is an optional linker amino acid sequence, A is an optional N-terminal amino acid sequence, B is an optional C-terminal amino acid sequence, and n is an integer greater than 1, and X is either: (a) an amino acid sequence provided as any one of SEQ ID NOs: 28 and 29; (b) an amino acid sequence having at least 70% sequence identity to any amino acid sequence from (a); or (c) an amino acid sequence comprising a functional fragment of at least 7 amino acids of any amino acid sequence from (a). Also disclosed is a hybrid protein having formula: NH2-A-[-X-L-]n-B-COOH wherein X is an amino acid sequence, L is an optional linker amino acid sequence, A is an optional N-terminal amino acid sequence, B is an optional C-terminal amino acid sequence, and n is an integer greater than 1, and wherein a first X moiety (-Xa-) has one of the following amino acid sequences: (a) an amino acid sequence provided as any one of SEQ ID NOs: 28 and 29; (b) an amino acid sequence having at least 70% sequence identity to any amino acid sequence from (a); or (c) an amino acid sequence comprising a functional fragment of at least 7 amino acids of any amino acid sequence from (a), and a second -X- moiety (-Xb-), wherein -Xb- has one of the following amino acid sequences: (d) SEQ IDs 32-477; (e) SEQ IDs 47 8-522; (f) SEQ IDs 523-2197; (g) SEQ IDs 2198-4357; (h) SEQ IDs 4358-4373; (i) SEQ ID 4374; (j) an amino acid sequence having at least 70% sequence identity to any amino acid sequence from (d) to (g); or (k) an amino acid sequence comprising a functional fragment of at least 7 amino acids of any amino acid sequence from (d) to (g). The proteins can be used in the manufacture of medicaments to treat Neisseria s.p. infections.