NOVEL ANTIOXIDATIVE COMPOUND ISOLATED FROM LEAVES OF CAMELLIA JAPONICA AND ISOLATION METHOD

摘要

A novel 3-hydroxy-4-methoxyphenyl-6-O-(4-hydroxylbenzoyl)-beta-D- glucopyranoside isolated from leaves of Camellia japonica is provided to show radical scavenging activity, thereby being used as an antioxidizing agent. A novel material having anti-oxidative activity is 3-hydroxy-4-methoxyphenyl-6-O-(4-hydroxylbenzoyl)-beta-D-glucopyranoside isolated by obtaining a hot-water extract of leaves of Camellia japonica, fractionating the extract into an aqueous solution fraction and an ethylacetate soluble neutral fraction using a buffer solution having a pH of 8.0(5% NaHCO3) and ethylacetate, purifying the ethylacetate soluble neutral fraction through a Sephadex LH-20 column chromatography to purify an active fraction and then isolating the desired product through HPLC using a methanol-water solvent system.