Labeling reagents, methods for synthesizing such reagents and methods for detecting biological molecules

摘要

The present invention relates to a temperature-stable labeling reagent of formula: in which R¹ represents H or an alkyl, aryl or substituted aryl group, R² represents a detectable marker or at least two detectable markers linked together by at least one multimeric structure, L is a linking arm containing a linear succession of at least two covalent bonds and n an integer equal to 0 or 1, R³ and R⁴ represent independently of each other: H, NO₂, Cl, Br, F, I, R²-(L)_n-Y-X-, OR, SR, NR₂, R, NHCOR, CONHR, COOR with R=alkyl or aryl, A is a linking arm containing at least one covalent double bond allowing conjugation of the diazo functional group with the aromatic ring and u is an integer between 0 and 2, preferably equal to 0 or 1, and -Y-X- represents -CONH-, -NHCO-, -CH₂O-, -CH₂S-. The present invention also describes a method for synthesizing said markers as well as applications for labeling biological molecules, in particular nucleic acids, with a labeling reagent carrying the diazomethyl functional group. The invention finds a preferred application in the field of diagnosis.