| 摘要 |
<p>A method for amplifying a nucleic acid from a microorganism is provided to perform a separation of the microorganism, a cell lysis and a nucleic acid amplification in one container, thereby capable of amplifying the nucleic acid conveniently and rapidly with high sensitivity. A method for amplifying a nucleic acid from a microorganism such as bacteria, fungi or virus comprises the steps of: (a) contacting a non-planar solid substrate with a blood culture material including the microorganism under pH of 3.0-6.0 to attach the microorganism to the solid substrate; (b) washing materials not attached to the solid substrate to remove the materials; and (c) subjecting the microorganism attached to the solid substrate to PCR, wherein all the steps are performed in a same container, the blood culture material includes polyanethole sulfonate, the sample is diluted by a phosphate buffer or an acetate buffer, and the solid substrate is selected from the group consisting of a solid substrate having a pillar structure where a plurality of pillar is formed, a bead-shaped solid substrate and a sieve-structured solid substrate, has a water contact angle of 70-95° and includes at least one amine-based functional group on the surface thereof.</p> |
