RAPID-THROUGHPUT TELEOST REGENERATION ASSAY

摘要

The present invention provides a method of simultaneously screening at least two candidate agents for an activity affecting regeneration of an embryonic teleost. According to this method, for each agent, a fin of the embryonic teleost is amputated. Next, the amputated teleost is incubated with the candidate agent for a specified period of time. After this period of time, the amputated teloeost is imaged. This image is compared to an image of an amputated teleost that was incubated for the specified period of time in the absence of the candidate agent. When comparing the images, a change in morphology of the amputated fin of the embryonic teleost incubated in the presence of the candidate agent compared to the amputated fin of the embryonic teleost raised in the absence of the candidate agent indicates that the candidate agent affects regeneration.