<p>In the systems and methods of the present invention a multifocal multiphoton imaging system a signal to noise ratio (SNR) that is reduced by over an order of magnitude al imaging depth equal to twice the mean free path scattering length of the specimen. An MMM system based on an area detector such as a multianode photomultiplier tube (MAPMT) that is optimized for high- speed tissue imaging. The specimen is raster- scanned with an array of excitation light beams. The emission photons from the array of excitation foci are collected simultaneously by a MAPMT and the signals from each anode are detected using high sensitivity, low noise single photon counting circuits. An image is formed by the temporal encoding of the integrated signal with a raster scanning pattern. A deconvolution procedure taking account of the spatial distribution and the raster temporal encoding of collected photons can be used to improve decay coefficient. We demonstrate MAPMT-based MMM can provide significantly better contrast than CCD- based existing systems.</p>
申请公布号
EP1889111(A2)
申请公布日期
2008.02.20
申请号
EP20060784478
申请日期
2006.05.25
申请人
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
发明人
BAHLMAN, KARSTEN;KIM, KI-HEAN;RAGAN, TIMOTHY;SO, PETER, T. C.