Cross-linked collagen matrix for producing a skin equivalent

摘要

A whole skin model is made by a process for the production of a whole skin model comprising the steps of: (a) providing a poorly soluble collagen obtained from collagen-containing tissue; (b) forming a homogeneous aqueous suspension by mixing the collagen with an aqueous medium; (c) forming a first matrix (A) by lyophilizing the collagen suspension; (d) forming a second matrix (B) by cross-linking the collagen in the first matrix (A) to form a mechanically stabilized second matrix (B); (e) sowing fibroblasts onto the second matrix (B) and allowing the fibroblasts to grow; (f) sowing keratinocytes onto the second matrix B and allowing the keratinocytes to grow; (g) further cultivating the fibroblasts and keratinocytes growing in and on matrix (B) to form a complete whole skin model comprised of a dermal and epidermal part.