发明名称 |
QUANTIFIZIERUNG VON BOTULINUM TOXIN |
摘要 |
The invention relates to a method for determining the quantity of pre-synaptic neuromuscular blocking substance (notably botulinum toxin) contained in a sample. In one aspects, the method comprises the following steps: (i) determining the minimum voltage Vm needed to induce the contraction of muscle tissue, said muscle tissue being connected to an electrical stimulator through a motor nerve and preferably immersed in an oxygenated physiological buffer containing glucose; (ii) adding the sample containing the pre-synaptic neuromuscular blocking substance; (iii) electrically stimulating, at a voltage at least equal to Vm, the muscle tissue at certain time intervals; (iv) comparing the effect induced by the sample to the effect induced by a reference substance and thereby determining the quantity of the pre-synaptic neuromuscular blocking substance in the sample. |
申请公布号 |
AT378602(T) |
申请公布日期 |
2007.11.15 |
申请号 |
AT20040713111T |
申请日期 |
2004.02.20 |
申请人 |
IPSEN LIMITED |
发明人 |
PICKETT, ANDREW MARTIN;QUIRK, ROBIN ANDREW;FRANCE, RICHARD MELVILLE;RICCALTON-BANKS, LISA ANNE |
分类号 |
G01N33/68;G01N33/50;G01N33/94 |
主分类号 |
G01N33/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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