发明名称 |
Process for producing recombinant polypeptides via a glycerol phosphate or sugar phosphate feed |
摘要 |
A process is described for producing a polypeptide heterologous to E. coli wherein E. coli cells comprising nucleic acid encoding the polypeptide are cultured in a culture medium while feeding to the culture medium a transportable organophosphate, such that the nucleic acid is expressed. The polypeptide is then recovered from the cells.
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申请公布号 |
US7294483(B2) |
申请公布日期 |
2007.11.13 |
申请号 |
US20050077272 |
申请日期 |
2005.03.10 |
申请人 |
GENENTECH, INC. |
发明人 |
LEUNG WOON-LAM SUSAN;SWARTZ JAMES R. |
分类号 |
C12N15/09;C07H21/04;C07K14/47;C07K14/65;C07K14/705;C07K16/00;C07K16/26;C12N1/20;C12N1/21;C12N1/32;C12N1/38;C12N15/70;C12N15/74;C12P21/00;C12P21/02;C12P21/06 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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