| 摘要 |
A monoclonal antibody and a diagnostic method of malaria parasites are provided to improve sensitivity, rapidness and detection amount of diagnosis by using the monoclonal antibody recognizing lactate dehydrogenase(LDH) of malaria parasites. A method for preparing the monoclonal antibody against malaria parasites comprises the steps of: (1) preparing a peptide for the specific site of LDH gene, labeling the MAPs(multiple antigen peptides) as a carrier to the peptide and immunizing a mouse with the peptide antigen; (2) separating serum from the immunized mouse and fusing it with myeloma; (3) selecting hybridoma cells from the fusion cell by using HAT(hypoxanthine, aminopterin and thymidine) medium, and isolating and culturing the selected hybridoma cells to confirm the antibody secretion; (4) selecting a monoclonal cell line producing the monoclonal antibody from the hybridoma cells by using limit dilution method; and (5) mass producing the monoclonal cell line and separating the monoclonal antibody from the cultured cell line by using protein A affinity chromatography, wherein the recombinant antigen has the amino acid sequence of SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3; the monoclonal antibody is B4D, C2B, D2H or D7E against the antigen of SEQ ID NO:2 or A4F, A7E, B9G or D5G against the antigen of SEQ ID NO:3; and the malaria parasite is Plasmodium falciparum or Plasmodium vivax. |
