Enzymatic modification in a continuously regenerated packed bed column

摘要

The invention relates to a process for the enzymatic modification of a substrate to produce a modified substrate which comprises the passing of the substrate through a packed bed column of a specific volume of immobilized enzyme with a flow of substrate in one direction and a periodic exchange of immobilized enzyme in the opposite direction. The substrate enters a packed bed column with a specific volume of immobilized enzyme at or near the bottom of the column and the modified substrate is discharged at or near the top of the column. A portion of the volume of the immobilized enzyme is periodically removed at the bottom of the column, while an equivalent portion of immobilized enzyme is periodically added at the top, thereby creating a flow of immobilized enzyme in the opposite direction of the substrate flow.