METHOD FOR PURIFYING GRANULOCYTE-COLONY STIMULATING FACTOR

摘要

The present invention provides a novel process for isolating and purifying rmetHuG-CSF from a G-CSF producing microorganism. The invention also relates to a method of improved over-expression of G-CSF in E. coll., a primer sequence for amplification of a modified hG-CSF sequence, plasmids, expression vectors and host cells for use in such an improved method.