发明名称 |
Hepatitis C Virus NS2/3 Activity Assay |
摘要 |
The invention provides a novel assay for NS2/3 cleavage detection, particularly, a novel assay for the detection of the NS3 cleavage product in the presence of uncleaved NS2/3 based on discrimination of the activity of the resulting NS3 protease product from the NS3 protease activity of the NS2/3 uncleaved protein. Certain reaction conditions allow this discrimination in situ without having to resort to physical separation of both proteases by decreasing the NS3 protease activity of the NS2/3 protein and increasing the NS3 protease activity of the resulting NS3 protease product, thereby producing a "signal window" upon which it is feasible to distinguish and measure NS3 protease activity produced from the cleavage of NS2/3.
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申请公布号 |
US2007212754(A1) |
申请公布日期 |
2007.09.13 |
申请号 |
US20060553531 |
申请日期 |
2006.10.27 |
申请人 |
MAURICE ROGER;THIBEAULT DIANE |
发明人 |
MAURICE ROGER;THIBEAULT DIANE |
分类号 |
C12Q1/70;C12P21/06;C12Q1/68 |
主分类号 |
C12Q1/70 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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