DNA POLYMERASE MUTANT

摘要

Disclosed is a novel thermostable family-D DNA polymerase (PolD) mutant which is thermostable, has a 3'-5' exonuclease activity (i.e., a proofreading function for DNA chain elongation), has a potent primer elongation activity, and can replicate a DNA region of interest within a short period even under high salt concentration conditions. The mutant can be produced from a thermostable heterooligomeric enzyme which is composed of a small subunit and a large subunit containing no intein sequence and has a DNA polymerase activity and a 3'-5' exonuclease activity, and can be produced by removing a DNA synthesis activity-regulating region from the small subunit in the thermostable heterooligomeric enzyme.