发明名称 |
METHODS AND MEANS FOR METABOLIC ENGINEERING AND IMPROVED PRODUCT FORMATION BY MICRO-ORGANISMS |
摘要 |
<p>The invention relates to the field of biochemistry, molecular biology and microbiology. More specific the invention relates to methods and means for metabolic engineering and improved product formation by a filamentous micro-organism or a low G+C gram-positive bacterium. The invention discloses that DasR and DasR binding sites play an important and universal role in the control of gene expression in micro-organisms Based on this finding, the invention provides multiple useful applications, such as a method for regulating the expression of a gene of interest, a method for controlling metabolism, a method for decreasing undesired expression and many more. Moreover, the invention also provides means that can be used to establish said methods: for example a micro-organism in which the DasR binding site in operable linkage with a particular gene has been modified to obtain increased or decreased expression of a protein (being a desired or undesired protein) encoded by said gene.</p> |
申请公布号 |
WO2007094667(A1) |
申请公布日期 |
2007.08.23 |
申请号 |
WO2007NL50061 |
申请日期 |
2007.02.14 |
申请人 |
UNIVERSITEIT LEIDEN;UNIVERSITY OF LIEGE;RIGALI, SEBASTIEN;TITGEMEYER, MANNFRED FRIEDRICH BRUNO;VAN WEZEL, GILLES PHILIPPUS |
发明人 |
RIGALI, SEBASTIEN;TITGEMEYER, MANNFRED FRIEDRICH BRUNO;VAN WEZEL, GILLES PHILIPPUS |
分类号 |
C12N15/09 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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