SELECTING AND STABILIZING DSRNA CONSTRUCTS

摘要

A method for managing crop pest resistance to an agent for controlling the crop pest. The crop pest is contacted with a nucleic acid segment produced by: (a) obtaining a starting nucleic acid molecule substantially complementary to a target gene; (b) preparing a plurality of segments from the starting nucleic acid molecule; (c) assaying the nucleic acid segments for the ability to suppress expression of the target gene when expressed as a dsRNA in a cell comprising the target gene; and (d) identifying at least a first nucleic acid segment from the plurality of segments that provides a desired level of suppression of the target gene when expressed as a dsRNA, and one additional agent selected from a patatin, a Bacillus thuringiensis insecticidal protein, a Xenorhabdus insecticidal protein, a Photorhabdus insecticidal protein, a Bacillus laterosporus insecticidal protein, a Bacillus sphaericus insecticidal protein, a biocontrol agent, and an insecticide.