ANTI-PRE-S1 HUMAN MONOCLONAL ANTIBODIES NEUTRALIZING HEPATITIS B VIRUS

摘要

A monoclonal antibody against pre-S1 of hepatitis B surface antigen is provided to overcome side effects on human body caused by chimeric antibodies and humanized antibodies derived from mouse antibodies and also overcome the difficulty of supplying raw materials of antibodies isolated from blood and infection by virus harmful to human body. The monoclonal antibody against pre-S1 of hepatitis B surface antigen is characterized in that it is prepared by a method comprising the steps of: (a) constructing a library human antibody fragment; (b) selecting an antibody fragment which specifically reacts on the pre-S1 of the hepatitis virus B through panning using a BIAcore from the library; and (c) identifying the neutralizing capability of the selected antibodies from the step(b) outside the body. The step(a) comprises the substeps of: (a-1) isolating lymphocyte from peripheral blood of 50 normal adults and purifying total RNA; (a-2) constructing a first strand cDNA from the RNA and performing polymerase chain reaction on human antibody gene; (a-3) preparing an electrocompetent cell; and (a-4) completing a single chain Fv(scFv) library.