METHOD FOR DETECTING 5-METHYLCYTOSINE

摘要

The present invention provides a method for the detection of 5-methylcytosine, characterized in that either a 5-methylcytosine specific binding moiety or a nucleotide analyte is attached to the tip of a single molecule force spectroscopy (SMFS) probe and the other of the 5-methylcytosine specific binding moiety or the nucleotide analyte is attached to a carrier, wherein the SMFS probe is brought into contact to the carrier and is detached and the detachment force is measured through an SMFS detection means; as well as an SMFS probe and an SMFS apparatus for said method.