Covalent attachment of ligands to nucleophilic proteins guided by non-covalent binding

摘要

A covalently reactive ligand analogue (CAL) of formula (1): wherein, L₁ . . . L_x . . . L_m are components defining a ligand determinant, L_x is a component unit of the ligand determinant selected from the group consisting of an amino acid residue, sugar residue, a fatty acid residue and a nucleotide, L' is a functional group of L_x, Y'' is atom, covalent bond or linker, Y' is an optional charged or neutral group Y is a covalently reactive electrophilic group that reacts specifically with a receptor that binds to said ligand determinant, and n is an integer from 1 to 1000 m is an integer from 1 to 30.